Describes the role of gas chromatography (GC) column quality control test probes (QC test probes), including ensuring that the columns have been properly deactivated, contain the correct amount of stationary phase, and have the same relative retention as the last column purchased.
This document introduces the Agilent J&W Ultra Inert GC column family, specifically the DB-5ms and HP-5ms models. It explains the importance of using specific Quality Control (QC) test probes to ensure column reproducibility and performance when analyzing chromatographically active analytes.
The Uber One mix includes ten probes: 1-propionic acid, 1-octene, n-octane, 4-picoline, n-nonane, trimethyl phosphate, 1,2-pentanediol, n-propylbenzene, 1-heptanol, and 3-octanone.
The test uses a 65 °C isothermal oven, a split injection of 0.02 µL with 1 ng each component, and FID detection at 325 °C.
The column has dimensions of 30 m x 0.25 mm x 0.25 µm.