This document mainly introduces the Agilent Genomics BioCel automated genomics system, which can automatically perform PCR preparation, unattended PCR and other tasks, thus improving the efficiency of genomic analysis.
This document provides a technical overview of the Agilent BioCel platform, an integrated preparative PCR system designed to create sequencer-ready sample plates from purified RNA or DNA with minimal user intervention. The system is capable of fully unattended operation for high-throughput genotyping applications.
The workflow utilizes single-step reverse transcription PCR and automated liquid handling to minimize plate processing times. Key features include spatial separation of liquid handling and thermal cycling, an enclosed clean environment, and quality assurance through barcode tracking. The platform is modular and customizable, supporting various configurations including optional thermal cycling cells and fluorescence/UV read-out capabilities.
| Throughput (Standard) | 20 samples per run with 192 clones amplified in 384-well plates in about 12 hours |
|---|---|
| Max Thermal Cyclers | Up to 18 thermal cyclers can be placed on the optional cell |
| RT-PCR Total Time | ~2.5 hours |
| Sequencing PCR Time | ~2.5 hours |
It is an integrated preparative PCR system capable of making sequencer-ready sample plates from purified RNA or DNA with the absolute minimum of user intervention.
The system utilizes single step RT-PCR and is designed around the Agencourt AMPure and CleanSEQ kits.
The enclosed clean system maintains a constant environment for better consistency, and plates are sealed/unsealed on demand to keep samples and reagents as clean as possible.