Agilent PLgel Column Solvent Transfer Technical Overview

About this manual

This document provides a technical overview of how solvent transfer procedures impact the efficiency of Agilent PLgel columns. It emphasizes that understanding these effects is crucial for selecting the optimal combination of column and solvent for specific applications.

The text includes a detailed procedure for testing column efficiency using different solvents (THF, DMF, acetone) and test probes (o-DCB, toluene). It presents data comparing the efficiency of two specific column types: PLgel 5 µm MIXED-C and PLgel 5 µm 100 Å, both with dimensions of 300 x 7.5 mm.

What's inside

• Introduction
• Solvent Transfer Effects
• Test Procedure
• Column Efficiency Data
• PLgel 5 µm MIXED-C
• PLgel 5 µm 100 Å

Specifications

Frequently asked questions

What is the flow rate used during the solvent transfer procedure?

The flow rate was maintained at 1.0 mL/min throughout the procedure.

How long should the column be flushed with acetone or DMF?

The column should be flushed for two column volumes at the specified flow rate.

What are the dimensions of the PLgel 5 ym MIXED-C column shown in the figure?

The column dimensions are 300 x 7.5 mm.