Agilent PLgel Polystyrene Calibrants Technical Overview

This document introduces the matching relationship between Agilent PLgel Individual Pore Size Columns and solvent. It introduces the characteristics of polystyrene standard and the elution curves under different solvent conditions.


Agilent PLgel Polystyrene Calibrants Technical Overview - cover page
Brand
Agilent
Document type
Product Leaflet
Language
English
Pages
2
File format
PDF
File size
541 KB
Published
05 July, 2012
Updated
30 September, 2023
MD5 checksum
3285F30115B51D0C7C3D0FFC44D2FA0B

About this manual

This document provides a technical overview of Agilent PLgel Individual Pore Size Columns, specifically focusing on the behavior of polystyrene calibrants in various solvents. It explains that while these calibrants exhibit true size exclusion behavior in tetrahydrofuran (THF), interactions may occur with more polar solvents.

What's inside

  • Introduction
  • Polystyrene Calibrants Behavior
  • Solvent Interactions
  • Adsorption Phenomenon
  • Calibration Curves

Frequently asked questions

In which solvent do polystyrene calibrants exhibit true size exclusion behavior?

Polystyrene calibrants exhibit true size exclusion behavior in tetrahydrofuran.

Why are polystyrenes unsuitable for use as GPC calibrants in polar organic eluents?

Some solute/stationary phase interaction occurs causing the elution times to increase and, for some standards, no elution at all.

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