This document describes the advantages and characteristics of the PL-SAX 1000Å material. This material is suitable for the analysis and purification of globular proteins, with the optimum pore size for maximum loading and low band broadening. PL-SAX is a hydrophilic strong anion exchange chromatographic packing material from Varian, Inc. The combination of the rigid macroporous polystyrene/divinylbenzene (PS/DVB) polymer matrix and chemically stable quaternized polyethyleneimine coating allows the analysis of biomolecules over a wide range of mobile phase conditions and pH. The physical stability of the media permits their use with high eluent flow rates and high speed gradients for very rapid separations. This excellent stability ensures both rapid equilibration between separations and the use of aggressive clean-up procedures employing high salt, NaOH, mineral and organic acids.
The Varian PL-SAX 1000A is a hydrophilic strong anion exchange chromatographic packing material designed for the analysis and purification of globular proteins. It features a rigid macroporous polystyrene/divinylbenzene (PS/DVB) polymer matrix with a chemically stable quaternized polyethyleneimine coating. This construction allows for analysis under a wide range of mobile phase conditions and pH levels, while maintaining physical stability for high eluent flow rates and high-speed gradients.
The material offers excellent stability, permitting rapid equilibration between separations and the use of aggressive cleanup procedures involving high salt, NaOH, mineral and organic acids, and a wide range of organic solvents. Its quaternary amine functionality enables anion exchange separations to be carried out at high pH. The document includes typical separation data for ovalbumin and soyabean trypsin inhibitor at various flow rates.
| Application | globular protein analysis and purification |
|---|---|
| Matrix Type | rigid macroporous polystyrene/divinylbenzene (PS/DVB) polymer matrix |
| Coating | chemically stable quaternized polyethyleneimine coating |
| Functionality | quaternary amine functionality |
| Flow Rate Capability | high eluent flow rates |
It is used for globular protein analysis and purification.
Yes, the quaternary amine functionality enables anion exchange separations to be carried out at high pH.
It permits the use of aggressive cleanup procedures employing high salt, NaOH, mineral and organic acids, and a wide range of organic solvents.