This document provides an optimized protocol for using the Stratagene Brilliant III Ultra-Fast QRT-PCR Master Mix with the QIAGEN Rotor-Gene Q Real-Time PCR Cycler. It includes instructions for preparing the reactions and using the reagent mixture.
This document serves as a quick reference guide for the Stratagene Brilliant III Ultra-Fast QRT-PCR Master Mix, specifically optimized for use with the QIAGEN Rotor-Gene Q Real-Time PCR Cycler. It provides an optimized protocol for experimental reaction preparation, including mixing reagents and adding RNA templates.
The guide details the steps for setting up the QPCR plate and thermal profile on the Rotor-Gene Q instrument, including specific cycling durations and temperatures. It also includes product information regarding catalog numbers and ordering details.
| Reaction Volume | 20 μl |
|---|---|
| Denaturation Temperature | 95°C |
| Denaturation Duration | 1 minute |
| Annealing/Extension Duration | 40 seconds |
| Catalog # (400 reactions) | 600884 |
| Catalog # (4000 reactions) | 600885 |
This guide provides an optimized protocol for using the Stratagene Brilliant III Ultra-Fast QRT-PCR Master Mix with the Rotor-Gene Q Real-Time PCR Cycler from QIAGEN.
The final reaction volume should be 20 μl, including the RNA.
For total RNA, the range is 0.1 pg – 100 ng, and for mRNA, it is 0.1 pg – 1 ng.