This quick reference guide provides an optimized protocol for using the Stratagene Brilliant III Ultra- Fast QPCR Master Mix with the Rotor- Gene Q Real-Time PCR Cycler from QIAGEN. For detailed instructions, refer to the full product manual.
This document serves as a quick reference guide for the Stratagene Brilliant III Ultra-Fast QPCR Master Mix, specifically detailing its use with the QIAGEN Rotor-Gene Q Real-Time PCR Cycler. It outlines the steps for preparing experimental reactions, including the required components and DNA quantities for genomic DNA and cDNA targets. The guide also provides instructions for setting up the QPCR plate and thermal profile within the instrument's software.
| Final reaction volume | 20 μl |
|---|---|
| Probe concentration range | 150–600 nM |
| Primer concentration range | 200–600 nM |
| Genomic DNA quantity range | 5 pg – 100 ng |
| cDNA quantity range | 0.1 pg – 100 ng |
| Number of cycles (cDNA) | 40 |
| Number of cycles (Genomic DNA) | 40 |
| Catalog # (400 reactions) | 600880 |
| Catalog # (4000 reactions) | 600881 |
It is optimized for use with the QIAGEN Rotor-Gene Q Real-Time PCR Cycler.
The recommended final reaction volume is 20 μl.
The suggested quantity range for genomic DNA is 5 pg to 100 ng per reaction.
The suggested quantity range for cDNA is 0.1 pg to 100 ng per reaction.