This quick reference guide provides an optimized protocol for using the Stratagene Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix with the Rotor-Gene Q Real-Time PCR Cycler from QIAGEN. For detailed instructions, refer to the full product manual.
This document serves as a quick reference guide for preparing reactions and setting up experiments using the Stratagene Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix. It details how to combine reagent components, including nuclease-free water, primers, and DNA templates, to achieve a final reaction volume of 20 μl.
| Final reaction volume | 20 μl |
|---|---|
| Primer concentration range | 200–500 nM |
| Genomic DNA input range | 5 pg – 25 ng |
| cDNA input range | 0.5 pg – 100 ng |
The protocol involves one cycle of 3 minutes at 95°C, followed by 40 cycles of 10 seconds at 95°C and 10 seconds at 60°C.
The protocol involves one cycle of 3 minutes at 95°C, followed by 40 cycles of 10 seconds at 95°C and 20 seconds at 60°C.