The document describes the Agilent Bioreagent components for processing microarrays. These include the Total RNA Isolation Mini Kit, Plant RNA Isolation Mini Kit, Low Input Quick Amp Labeling Kit (one-color/two-color/no dye), and Quick Amp Labeling Kit (one-color). These kits are used for isolating total RNA from mammalian cells, tissues, yeast, and bacteria, and for amplifying and labeling RNA for oligo microarray analysis.
This document serves as a product catalog for Agilent Bioreagent Components designed for processing microarrays. It details a wide range of reagents for gene expression and miRNA analysis, including Total RNA Isolation Kits, Quick Amp Labeling Kits, and various Spike-in Kits for control purposes.
The catalog also covers reagents for CGH, ChIP-on-Chip, and DNA Methylation applications. Key offerings include Genomic DNA Enzymatic Labeling Kits, ULS Labeling Kits, and specific Hybridization and Wash Buffers optimized for different processing protocols, such as manual or Tecan processing.
| Low Input Quick Amp Labeling Kit input | 25 — 200 ng total RNA input |
|---|---|
| Quick Amp Labeling Kit, two-color reactions | 48 reactions |
| Gene Expression Hybridization Kit slides | at least 10 slides |
| Gene Expression Wash Buffer Kit capacity | up to 80 slides |
| miRNA Complete Labeling and Hybridization Solution capacity | 24 microarrays |
| Universal miRNA Reference Kit contents | 9 different tissues and cell lines |
| Universal Human Reference RNA coverage | human, mouse and rat |
| Agilent RNA 6000 Nano Kit sample capacity | 300 samples |
| Agilent DNA 1000 Kit fragment range | 25 to 1000bp |
It uses 25 — 200 ng total RNA input.
The kit is sufficient for 48 reactions, 24 with each dye.
It is sufficient for at least 10 slides.
No, it is not for use with miRNA experiments.