Provides a quick reference guide for using Stratagene Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix with the 7500 Fast Real-Time PCR System, including preparing reactions, reagent mixture, reaction mixture and more.
This document serves as a quick reference guide for the Stratagene Brilliant III Ultra-Fast SYBR Green QPCR Master Mix, specifically optimized for use with the ABI 7500 Fast Real-Time PCR System from Applied Biosystems. It outlines the steps for preparing reactions, including diluting the reference dye and combining reagent components to a final volume of 20 μl. The guide also details the setup of the QPCR plate and thermal profile within the 7500 software, recommending the selection of SYBR Green Reagents and Fast ramp speed.
| Reaction Volume | 20 μl |
|---|---|
| Primer Concentration | 200–500 nM |
| Reference Dye Dilution | 1:500 |
| Catalog #400 Reactions | 600 805 |
| Catalog #4000 Reactions | 600 816 |
It is optimized for use with the 7500 Fast Real-Time PCR System from Applied Biosystems.
The reference dye should be diluted 1:500 using nuclease-free PCR-grade water.
The recommended reaction volume is 20 μl.