This application note describes a method for peptide mapping of monoclonal antibodies using the Agilent Poroshell 120 SB-C18 column. The method achieves 100% sequence coverage in 20 minutes.
This document describes a method for peptide mapping of monoclonal antibodies (mAb) using the Agilent Poroshell 120-SB C18 column coupled with LC-MS. The goal is to achieve 100% sequence coverage of both heavy and light chains while significantly reducing analysis time compared to traditional protocols.
| Column Dimensions | 3.0 X 150 mm |
|---|---|
| Column Part Number | PN 683975-302 |
| Column Temperature | 40 °C |
| Flow Rate | 0.3 mL/min |
| Total Analysis Time | 20 min |
The total analysis time, including gradient re-equilibration, was only 20 minutes.
Yes, the method resulted in 100% sequence coverage for both the heavy and light chains of the mAb.