Agilent RNA LabChip assays are used for fast, efficient quality control of RNA. The assay kit can be used in nucleic acid microarray experiments to control the quality of RNA, to quantify RNA, and to label RNA samples.
This document outlines Agilent's sample preparation solutions designed to ensure meaningful microarray results. It highlights the use of Agilent RNA LabChip Assays and the 2100 bioanalyzer for quality control to verify RNA integrity. The text also details how starting with less RNA can be sufficient for labeling experiments using specific Agilent Labeling Kits.
| Minimum total RNA input for Fluorescent Linear Amplification Kit | 50 ng |
|---|---|
| Run time for Fluorescent Linear Amplification Kit (eRNA) | 6 hours |
| Run time for Fluorescent Linear Amplification Kit (cDNA) | 10 hours |
| Amplification fold for Fluorescent Linear Amplification Kit | 200-300 fold |
| Minimum sample input for Fluorescent Direct Label Kit | 10 pg total RNA |
| Sensitivity for Fluorescent Direct Label Kit | 3 mANA copies per 10° cells |
| Qualitative range for ANA 6000 Nano Kit | 5-500 ng/pl total RNA |
| Qualitative range for RNA 6000 Pico Kit | 200-5000 pg/ul total ANA |
The kit requires as little as 50 ng total RNA.
The procedure takes only 6 hours for eRNA.
It has a sensitivity of 3 mANA copies per 10° cells on Agilent's microarrays.