This document describes the use of short, small-particle columns for fast and efficient separations of herbicide mixtures. By using a 3.5µm particle column, similar efficiency can be achieved in a shorter time compared to columns with 5µm particles and twice the length. Additionally, increasing the flow rate on the 3.5µm, 75 mm column further reduces the run-time, with efficiencies nearly equivalent to those of the 5µm 150 mm column run at a slower flow rate.
This document presents data for the Hewlett-Packard ZORBAX SB-C8 Injection column, specifically highlighting the performance of short, small-particle columns for fast and efficient separations. It details a chromatographic separation of a herbicide mixture using a 4.6 x 75 mm column with 3.5 µm particles.
| Column Dimensions | 4.6 x 75 mm |
|---|---|
| Particle Size | 3.5 µm |
| Mobile Phase A | H2O to pH 2.0 with TFA |
| Mobile Phase B | ACN |
| Detection | UV (254 nm) |
| Temperature | 35°C |
They enable high-speed, high-resolution separation of a herbicide mixture in less than 8 minutes.
Smaller particle size (3.5m) leads to separations of similar efficiency when compared to 5um particles in columns of twice the length.