This document discusses the effect of organic modifier and pH on the selectivity of Zorbax SB-CN column. pH control is crucial for providing separation stability for polar organic compounds that are basic or acidic. The short-chain SB-CN column is highly stable under low pH conditions (e.g. pH 2.5).
This document provides data for the Hewlett-Packard Zorbax SB-CN, 4.6 x 150 mm column (part number 883975.905). The analysis was conducted using a 25 mM phosphate buffer at a flow rate of 1.0 mL/min and a temperature of 22°C.
The document highlights that pH control is very important for providing separation stability for polar organic compounds that are basic or acidic. It notes that the short-chain SB-CN column is very stable under low pH conditions, such as pH 2.5.
| Column Dimensions | 4.6 x 150 mm |
|---|---|
| Part Number | 883975.905 |
| Buffer Concentration | 25 mM phosphate buffer |
| Flow Rate | 1.0 mL/min |
Yes, the short-chain SB-CN column is very stable under low pH conditions, such as pH 2.5.
pH control is very important in providing separation stability for polar organic compounds that are basic or acidic.