ZORBAX SB-C18 (3.0 x 250 mm) (P/N: 880975.302) Mobile Phase: A: 0.01% Acetic Acid in H20 B: Acetonitrile 0.01% Acetic Acid Injection volume 50 l 0.5 mL/min 40°C Detect. UV( 230 270

About this manual

This document presents a chromatogram showing the high-resolution separation of seven sulfonylurea pesticide metabolites. The analysis was performed using a ZORBAX SB-C18 (3.0 x 250 mm) column, identified by part number 880975.302.

The document details the specific experimental conditions used to achieve this separation, including mobile phase composition (0.01% Acetic Acid in H2O and Acetonitrile), flow rate (0.5 mL/min), temperature (40°C), and a detailed gradient elution profile. It also provides instructions for sample preparation via solid-phase extraction.

What's inside

• High-Resolution Separation
• Sulfonylurea Pesticides
• Chromatogram Data
• Mobile Phase Conditions
• Gradient Elution Profile
• Sample Preparation
• Solid-Phase Extraction

Specifications

Frequently asked questions

What is the mobile phase composition for this separation?

Mobile Phase A is 0.01% Acetic Acid in H2O and Mobile Phase B is Acetonitrile with 0.01% Acetic Acid.

What is the injection volume and flow rate for this separation?

The procedure requires an injection volume of 50ul and a flow rate of 0.5 mL/min.

What is the maximum sample passing rate for solid-phase extraction?

Samples must be passed through the cartridge at a rate not exceeding 500 mi/hr.