This Application Note describes the synthesis of d(T)70 using Varian StratoSpheres DNA.
The document details an experiment synthesizing a poly-T oligomer (d(T)70) on a 0.2 UM scale using an Applied Biosystems 392 DNA/RNA Synthesizer and standard chemistry. The synthesis was performed to reveal information about the pore structure and accessibility of the support material.
Following synthesis, the oligonucleotides were cleaved with ammonium hydroxide and analyzed via HPLC. The results demonstrate that the StratoSpheres DNA dT support performs equally well compared to leading commercial macroporous PS/DVB supports, whereas controlled pore glass (CPG) support showed compromised yields due to incomplete coupling reactions.
The synthesis reveals information about the pore structure and pore accessibility of the support during synthesis.
The flow rate is set to 1.5 mL/min.