This application note describes the steps for synthesizing a bradykinin analog using AmphiSpheres resins
This document details the synthesis of a C-terminal amide analog of bradykinin using AmphiSpheres resins. The analog is a nine amino acid peptide containing two arginine residues, prepared using Pbf side chain protection. The synthesis was performed on a 0.1 mmol scale using a peptide synthesizer with Fmoc-AA-OH and HBTU coupling reagents.
| Column | Varian Polaris 5 ym C18-A 150 x 4.6 mm |
|---|---|
| Gradient | 5 — 95% MeCN (0.1% TFA), 0 - 30 min |
| Flow rate | 1 mL/min |
| UV detector | 220 nm |
| Injection volume | 20 uL |
| Peptide scale | 0.1 mmol |
| Coupling reagent | HBTU |
| Coupling time | 9 minute |
The synthesis uses AmphiSpheres resins.
The analog is H-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-NH2.
The peptide is cleaved by treating with TFA / TIPS / water 95:2.5:2.5 (v/v) for 2-3h.