This application note describes an automated analysis method for determining the uniformity of dosage in quinine sulfate tablets using an Agilent fiber-optic autosampler. The method uses a Cary 3C double beam UV/Vis spectrophotometer, Agilent SPS-5 autosampler, Cary fiber probe, and Cary fiber optic coupler. A Cary OS2 concentration application module and simple ADL program are used to locate the peak maximum and its absorbance value, from which the concentration of the analyte can be determined from known standards or a known extinction coefficient. This method has been applied to the measurement of uniformity of dosage for quinine sulfate in tablets using the USP procedure.
This document details an analytical procedure for measuring the uniformity of dosage in quinine sulfate tablets. It utilizes the Agilent SPS-5 Autosampler coupled with a Cary 3C Double Beam UV/Vis Spectrophotometer and the Cary 082 Concentration Application module. The method employs a specific ADL program to locate peak maximum absorbance within a defined range, ensuring accurate concentration determination from known standards or extinction coefficients.
| Wavelength scan range | 400 to 320 nm |
|---|---|
| Correlation coefficient | 0.99998 |
| Standard deviation | 0.02 |
The Cary 082 Concentration Application module with Cary OS2 software is used.
The peak maximum must occur between 320 nm and 360 nm.
Ten tablets are selected and transferred to individual volumetric flasks.