This guide shows how to use the Agilent 7000A Triple Quad GC/MS to acquire and analyze sample data.
This document serves as a familiarization guide for the Agilent MassHunter Workstation Software, specifically focusing on Data Acquisition for the Agilent 7000A Triple Quad GC/MS. It instructs users on how to prepare their system and samples before beginning data acquisition exercises.
The guide walks users through developing an acquisition method by setting inlet and injection parameters, entering GC acquisition conditions, creating methods for finding precursor and product ions, and establishing MRM (Multiple Reaction Monitoring) methods. It also covers the creation of quantitative analysis batches and methods to quantitate unknown samples.
| Column Type | Agilent HP-5MS 5% Phenyl Methyl Siloxane: 30 m x 250 µm x 0.25 µm |
|---|---|
| Sample Concentration (Qualitative) | 10 ng/µL |
| Sample Concentration (Quantitative Calibration) | 1, 0.5, 0.25, 0.125, and 0.0625 ng/µL |
| Sample Concentration (Unknowns) | 0.4, 0.2, and 0.1 ng/µL |
| Oven Equilibration Time | 0.5 min |
You can use the demo data files located in the Benzodiazepine directory shipped with the system.
A 1-mL ampoule of a Benzodiazepine mix sample (Agilent part number B-033) diluted to 10 ng/µL.
The Agilent HP-5MS 5% Phenyl Methyl Siloxane: 30 m x 250 µm x 0.25 µm column.