Agilent 5188-2750 Peptide Cleanup C18 Spin Tubes are used for purification and concentration of peptide samples. Each C18 spin tube contains 8 mg of C18 resin and can process sample volumes from 10 to 150 μl. This product is recommended for cleanup of peptides from in-solution digests.
The document describes the Agilent 5188-2750 Peptide Cleanup C18 Spin Tubes, a kit containing fifty spin tubes with C18 resin designed to remove interfering contaminants from peptide samples. These tubes are recommended for processing digests derived from up to 30 µg of total peptide or 20 ng of protein, facilitating cleanup for MALDI-MS and ESI-MS applications.
| Tube Count | Fifty (50) Peptide Cleanup Spin Tubes |
|---|---|
| Resin Amount per Tube | 8 mg |
| Sample Volume Range | 10 to 150 µl |
| Protein Capacity | 20 ng |
| Peptide Capacity | 30 µg |
Store at room temperature upon receipt.
Yes, methanol can be substituted for ACN in the activation solution.
Dry the sample in a vacuum evaporator and resuspend it in 20-50 µl of 0.5% TFA in 5% ACN.