This document provides instructions for using ArcticExpress RIL Competent Cells and ArcticExpress RP Competent Cells, including product features and characteristics.
The document is an instruction manual for the ArcticExpress RIL and ArcticExpress RP Competent Cells (Catalog #230193-#230196). These cells are engineered with CodonPlus technology to address protein insolubility and codon bias. They are derived from the BL21-Gold cell line and feature cold-adapted chaperonins (Cpn10 and Cpn60) from the psychrophilic bacterium Oleispira antarctica to improve protein folding at low temperatures.
| Efficiency (ArcticExpress RIL/RP) | 25 x 10^8 cfu/ug of pUC18 DNA |
|---|---|
| Storage Temperature | -80°C |
| Tube Color (RIL) | Yellow tubes |
| Tube Color (RP) | Green tubes |
Store cells immediately at -80°C. Do not place the cells in liquid nitrogen.
A low-temperature (10-13°C) incubator with shaker and 14-ml BD Falcon polypropylene round-bottom tubes are required.
They co-express the cold-adapted chaperonins Cpn10 and Cpn60 from the psychrophilic bacterium Oleispira antarctica.