This document provides instructions for using the QuikChange II XL Site-Directed Mutagenesis Kit (catalog #200521 and #200522). The kit is intended for research use only and is not for use in diagnostic procedures. The document includes ordering information and technical service contact details, as well as a list of the materials provided and additional materials required. Additionally, the document provides guidelines and considerations for mutagenic primer design.
The QuikChange II XL Site-Directed Mutagenesis Kit is designed for in vitro site-directed mutagenesis, allowing users to make point mutations, replace amino acids, or delete/insert sequences in virtually any double-stranded plasmid. The method eliminates the need for subcloning and single-stranded DNA templates by utilizing a rapid three-step procedure involving thermal cycling, Dpn I digestion, and transformation.
| Catalog #200521 reactions | 10 reactions |
|---|---|
| Catalog #200522 reactions | 30 reactions |
The kit is used to make point mutations, replace amino acids, and delete or insert single or multiple adjacent amino acids.
The basic procedure utilizes a supercoiled double-stranded DNA (dsDNA) vector with an insert of interest.
Plasmid DNA isolated from dam- E. coli strains, including JM110 and SCS110, is not suitable.
The Dpn I endonuclease targets the sequence 3°-GmATC-37.