QuikChange Site-Directed Mutagenesis Kit instruction manual, introduces the usage methods, precautions, etc. of the product
The QuikChange Site-Directed Mutagenesis Kit allows researchers to introduce specific point mutations, amino acid switches, or insertions/deletions into virtually any double-stranded plasmid. The system utilizes PfuTurbo DNA polymerase and a temperature cycler to generate mutants with greater than 80% efficiency without requiring specialized vectors or subcloning steps.
| Catalog Number (30 reactions) | 200518 |
|---|---|
| PfuTurbo DNA polymerase activity per reaction | 2.5 U/ul |
| Dpn I restriction enzyme activity per reaction | 10 U/ul |
The protocol generates mutants with greater than 80% efficiency.
XL1-Blue Supercompetent Cells and pUC18 Control Plasmid must be stored at -80°C.