This manual introduces the use of Agilent mAb-Glyco Chip
The Agilent mAb-Glyco Chip is designed for the on-chip deglycosylation of monoclonal antibodies (mAbs) followed by enrichment, separation, and mass spectrometry-based detection of cleaved N-glycans. The process utilizes an integrated immobilized PNGase F enzyme reactor column within the chip system.
This manual covers system preparation including hardware modifications such as configuring the Chip Cube in backflush mode and installing the Enablement Kit. It details reagent preparation, operating procedures for chromatographic checkout and functional checks, storage guidelines, MS maintenance, automated data analysis using Mass Hunter software, and a troubleshooting guide.
| Application | on-chip deglycosylation of monoclonal antibodies (mAbs) |
|---|---|
| Detection Method | MS based detection of cleaved N-glycans |
| Enzyme Technology | integrated immobilized PNGase F enzyme reactor column |
The Chip Cube must be configured in backflush mode to transfer cleaved glycans onto the PGC enrichment column.
Operating the chip without the installed enablement kit can lead to a significant decrease in chip lifetime.
The System Conditioning Reagent, which is lyophilized, must be stored at -20°C.