This quick reference guide provides an optimized protocol for using the Stratagene Brilliant III Ultra- Fast SYBR® Green QPCR Master Mix with the LightCycler 480 Real-Time PCR System from Roche.
The document outlines a quick reference guide for preparing reactions and setting up experiments using the Stratagene Brilliant III Ultra-Fast SYBR Green QPCR Master Mix. It details the steps to combine reagents, including nuclease-free water, master mix, primers, and DNA templates, to achieve a final reaction volume of 20 μl.
The suggested quantity range brings the final reaction volume to 20 μl.
The optimized concentration for both upstream and downstream primers is between 200–500 nM.
The Detection Format should be set to SYBR Green I/HRM Dye.