Perfect Match PCR Enhancer instruction manual
This document provides instructions for the use of Agilent Perfect Match PCR Enhancer, a reagent designed to increase the specificity and yield of primary PCR amplification products. It is intended for research use only and not for diagnostic procedures.
The manual outlines storage conditions at -20°C and provides specific optimization guidelines based on template type (plasmid vs. genomic). Detailed protocols are included for preparing the reaction mixture, thermal cycling parameters, and troubleshooting common issues such as low yield or false products.
| Catalog Numbers | #600129 (100 U) and #600130 (200 U) |
|---|---|
| Storage Temperature | -20°C |
| Concentration | 1 U/μl |
No, it is for research use only and not for use in diagnostic procedures.
Use 1 U of Perfect Match PCR enhancer per μg of DNA template.
Use 1 U of Perfect Match PCR enhancer per 100 ng of DNA template.