This document provides an optimized protocol for using the Stratagene Brilliant III Ultra-Fast QPCR Master Mix with the LightCycler® 480 Real-Time PCR System.
This document provides a quick reference guide for preparing reactions and setting up experiments using the Stratagene Brilliant III Ultra-Fast QPCR Master Mix with the LightCycler 480 Real-Time PCR System. It details the steps to prepare reagent mixtures, including specific quantities of water, master mix, probes, and primers required to achieve a final reaction volume of 20 μl.
The suggested quantity range brings the final reaction volume to 20 μl.
The optimized concentration for the experimental probe is between 150–600 nM.
The optimized concentration for both upstream and downstream primers is between 200–600 nM.