The kit can be used to detect IL-6 in rat serum, plasma, cell culture supernatant or other related biological fluids.
This manual describes the rat interleukin‑6 (IL‑6) ELISA immunoassay kit, intended for research use only. It lists the kit components, storage conditions, and the detection range of 0.312 pg/ml – 20 pg/ml with a minimum detection limit of 0.078 pg/ml. The kit detects both natural and recombinant rat IL‑6 without cross‑reaction to other proteins.
The document provides detailed procedures for sample collection (serum, plasma, cell culture supernatant), preparation of standards and reagents, step‑by‑step assay incubation at 37 °C, washing, substrate development, and calculation of concentrations using a standard curve. Important precautions such as avoiding sample hemolysis, proper washing to prevent false positives, and storage of reagents are also covered. This manual is written in Chinese.
| Detection range | 0.312 pg/ml – 20 pg/ml |
|---|---|
| Specificity | No cross‑reaction with other related proteins |
| Effective shelf life | 6 months |
| Molecular weight of IL‑6 | 26 kDa |
| Amino‑acid length of IL‑6 | 184 residues |
| Recommended storage temperature | -20 °C (long term) or 2‑8 °C (short term) |
The kit can measure IL‑6 concentrations from 0.312 pg/ml up to 20 pg/ml.
Store the kit at –20 °C for long‑term storage, or at 2‑8 °C if it will be used within a short period.
Allow whole blood to clot at room temperature for 2 h or at 4 °C overnight, then centrifuge at 1000 × g for 20 min and collect the supernatant. Store the serum at –20 °C or –80 °C, avoiding repeated freeze‑thaw cycles.