Key facts
- Manuals
- 4
- Categories
- 1
- Languages
- ZH · JA
- Last added
- 2012-02-04
Featured rat interleukin manuals
Rat IL-6 ELISA Immunoassay Kit User Manual
This manual describes the rat interleukin‑6 (IL‑6) ELISA immunoassay kit, intended for research use only. It lists the kit components, storage conditions, and the detection range of 0.312 pg/ml – 20 pg/ml with a minimum detection limit of 0.078 pg/ml. The kit detects both natural and recombinant rat IL‑6 without cross‑reaction to other proteins.
The document provides detailed procedures for sample collection (serum, plasma, cell culture supernatant), preparation of standards and reagents, step‑by‑step assay incubation at 37 °C, washing, substrate development, and calculation of concentrations using a standard curve. Important precautions such as avoiding sample hemolysis, proper washing to prevent false positives, and storage of reagents are also covered. This manual is written in Chinese.
Covers: Detection Range and Sensitivity · Reagents and Kit Components · Sample Collection and Storage · Standard and Sample Preparation · Assay Procedure · Washing and Substrate Development
rat interleukin 4(IL-4) elisa immunoassay kit Manual
This manual provides detailed guidance for the rat Interleukin‑4 (IL‑4) ELISA immunoassay kit, covering kit components, storage conditions, and preparation of standards and samples. It explains the principle of the sandwich ELISA, the use of biotin‑labeled antibodies, HRP‑avidin conjugate, and TMB substrate to generate a measurable color change.
Step‑by‑step procedures describe plate loading, incubation times, washing steps, and addition of stop solution, followed by absorbance measurement at 450 nm. The document also includes notes on sample handling, troubleshooting tips, and calculation of IL‑4 concentrations from standard curves. This manual is written in Japanese.
Covers: Kit Components · Assay Principle · Reagent Preparation · Assay Procedure · Plate Washing · Data Acquisition
IL-21 Rat ELISA Kit User Manual
This manual describes the rat interleukin‑21 (IL‑21) ELISA immunoassay kit, intended for quantitative measurement of IL‑21 in serum, plasma, cell culture supernatants and other biological fluids. It outlines the detection range, limit of detection, specificity, shelf life, and recommended storage conditions.
The document provides detailed procedures for sample preparation, reagent preparation, plate loading, incubation, washing, substrate development, stop solution addition, plate reading, and calculation of concentrations. It also lists required equipment, precautions, and tips for accurate results. This manual is written in Chinese.
Covers: Detection Range and Sensitivity · Storage and Shelf Life · Reagent Composition · Required Equipment · Sample Collection and Preparation · Standard and Antibody Dilution
rat interleukin 1β(IL-1β) elisa immunoassay kit Manual
This manual describes the rat interleukin‑1β (IL‑1β) ELISA immunoassay kit, intended for research use only. It lists the detection range (3.12 pg/ml – 200 pg/ml), minimum detection limit (0.78 pg/ml), and specificity for native or recombinant rat IL‑1β without cross‑reaction.
The document provides detailed procedures for sample collection, reagent preparation, assay execution on a 96‑well plate, washing methods, calculation of concentrations, and safety precautions. Storage conditions for reagents and samples, as well as recommended equipment, are also included. This manual is written in Chinese.
Covers: Detection Range and Sensitivity · Specificity · Reagent Storage · Sample Collection and Preservation · Reagent Preparation · Assay Procedure
Latest rat interleukin manuals
Rat IL-6 ELISA Immunoassay Kit User Manual
User Manual 6 pages · 128 KB · ZHInstructions for using the rat interleukin‑6 ELISA kit, including reagents, sample handling, assay steps, and data calculation.
rat interleukin 4(IL-4) elisa immunoassay kit Manual
User Manual 6 pages · 126 KB · JAInstructions for using the rat IL‑4 ELISA kit, including storage, sample preparation, assay procedure, and data analysis.
IL-21 Rat ELISA Kit User Manual
User Manual 5 pages · 115 KB · ZHInstructions for using the rat IL‑21 ELISA kit, including storage, reagents, assay steps and data analysis.
rat interleukin 1β(IL-1β) elisa immunoassay kit Manual
User Manual 6 pages · 128 KB · ZHInstructions for using the rat IL‑1β ELISA kit, including storage, sample handling, assay steps, and data calculation.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in rat interleukin manuals
What is the detection range of the rat IL‑6 ELISA kit?
The kit can measure IL‑6 concentrations from 0.312 pg/ml up to 20 pg/ml.
How should the kit be stored?
Store the kit at –20 °C for long‑term storage, or at 2‑8 °C if it will be used within a short period.
What is the detection range of the IL‑4 ELISA kit?
The kit can detect IL‑4 concentrations from 0.78 ng/ml up to 30 ng/ml.
How should the kit be stored?
Store the kit at –20 °C for long‑term storage, or at 2‑8 °C for short‑term use.
How should the kit be stored before use?
Store the kit at –20 °C for long‑term storage, or at 2‑8 °C for short‑term use.
What equipment is required to run the assay?
A standard ELISA plate reader, a high‑speed centrifuge, an incubator, clean tubes and a multichannel pipette are needed.