This kit can be used to quantitatively determine the content of IL-21 in rat serum, plasma, cell culture supernatant or other related biological fluids.
This manual describes the rat interleukin‑21 (IL‑21) ELISA immunoassay kit, intended for quantitative measurement of IL‑21 in serum, plasma, cell culture supernatants and other biological fluids. It outlines the detection range, limit of detection, specificity, shelf life, and recommended storage conditions.
The document provides detailed procedures for sample preparation, reagent preparation, plate loading, incubation, washing, substrate development, stop solution addition, plate reading, and calculation of concentrations. It also lists required equipment, precautions, and tips for accurate results. This manual is written in Chinese.
| Shelf life | 6 months |
|---|---|
| Long‑term storage temperature | -20 °C |
| Short‑term storage temperature | 2‑8 °C |
Store the kit at –20 °C for long‑term storage, or at 2‑8 °C for short‑term use.
A standard ELISA plate reader, a high‑speed centrifuge, an incubator, clean tubes and a multichannel pipette are needed.
Samples are incubated at 37 °C for 120 minutes during the first step, and subsequent incubation steps are 60 minutes each.