Rat Interleukin-4 (IL-4) ELISA Kit is intended for the quantitative determination of IL-4 in serum, plasma, cell culture supernatants or other relevant biological fluids of rats.
This manual provides detailed guidance for the rat Interleukin‑4 (IL‑4) ELISA immunoassay kit, covering kit components, storage conditions, and preparation of standards and samples. It explains the principle of the sandwich ELISA, the use of biotin‑labeled antibodies, HRP‑avidin conjugate, and TMB substrate to generate a measurable color change.
Step‑by‑step procedures describe plate loading, incubation times, washing steps, and addition of stop solution, followed by absorbance measurement at 450 nm. The document also includes notes on sample handling, troubleshooting tips, and calculation of IL‑4 concentrations from standard curves. This manual is written in Japanese.
| Detection range | 0.78 ng/ml – 30 ng/ml |
|---|---|
| Lower limit of detection | 0.195 ng/ml |
| Storage temperature | -20 °C (long‑term) or 2‑8 °C (short‑term) |
| Measurement wavelength | 450 nm |
The kit can detect IL‑4 concentrations from 0.78 ng/ml up to 30 ng/ml.
Store the kit at –20 °C for long‑term storage, or at 2‑8 °C for short‑term use.
Read the absorbance at 450 nm after adding the stop solution.