This kit can be used to detect the content of IL-1β in rat serum, plasma, cell culture supernatant or other relevant biological fluids.
This manual describes the rat interleukin‑1β (IL‑1β) ELISA immunoassay kit, intended for research use only. It lists the detection range (3.12 pg/ml – 200 pg/ml), minimum detection limit (0.78 pg/ml), and specificity for native or recombinant rat IL‑1β without cross‑reaction.
The document provides detailed procedures for sample collection, reagent preparation, assay execution on a 96‑well plate, washing methods, calculation of concentrations, and safety precautions. Storage conditions for reagents and samples, as well as recommended equipment, are also included. This manual is written in Chinese.
| Detection range | 3.12 pg/ml – 200 pg/ml |
|---|---|
| Minimum detection limit | 0.78 pg/ml |
| Specificity | Detects rat IL‑1β only, no cross‑reaction with related proteins |
| Shelf life | 6 months |
| Assay format | 96‑well microplate |
The kit can measure concentrations from 3.12 pg/ml up to 200 pg/ml.
Serum can be kept at room temperature for 2 hours or at 4‑8 °C overnight, then centrifuged; for longer storage, freeze at –20 °C or –80 °C, avoiding repeated freeze‑thaw cycles.
Plot a standard curve using the log of standard concentrations versus OD values, then determine sample concentrations from the curve and multiply by the dilution factor.