This document describes an approach to evaluate an Ion S5 ™ or Ion GeneStudio ™ S5 sequencing run report. Although each sequencing run should be evaluated as a whole, there are individual metrics that can help you assess a sequencing run. This document provides an overall view of expected run performance. For cases that involve further help, contact Thermo Fisher Scientific Technical Support at www.thermofisher.com/support, or your contract support personnel.