Agilent Manuals

This document describes the method of analyzing pharmaceuticals in water samples using the Agilent G6410A triple quadrupole mass spectrometer (QQQ) with HPLC/MS/MS. The method was developed following the guidelines of EPA method 1694. Four different chromatographic gradients and LC conditions were used to accommodate the polarity and systematics of various pharmaceuticals. Additionally, analysis using positive and negative ion electrospray was performed for quantification and qualification of each compound.

This document describes a simple isocratic method for analyzing artemisinin using the Agilent 1200SL system with high-speed separation high-throughput (RRHT) columns and ChromSword software. The method development was automated using the ChromSword software, which helped in selecting the optimal column. By utilizing a fully automated unmanned method development program, the analysis time was significantly reduced.

This document introduces a new method that combines immunoprecipitation with Agilent High Sensitivity Protein 250 assay on Agilent 2100 Bioanalyzer to detect proteins on a microchip with high specificity and sensitivity. This method is useful for protein expression and purification research in fields such as pharmaceuticals, biotechnology, and specialized research institutions. Compared to Western blotting, this method offers more reliable results, better accuracy, improved productivity, reduced antibody costs, and lower reagent consumption.

This application note describes some of the key points and advice for using the auto-backflush function on the Agilent 7890A/5975A GC/MS system, and provides an example of how to set up and use backflushing for monitoring biomarkers in crude oil.

This application note describes a 6-minute Agilent 7890A GC-based refinery gas analysis (RGA) system. The system consists of 7890A GC, three detectors (TCD, ECD and FID), five valves, a 10-port valve and seven columns. The system can fully analyze nC6 and below hydrocarbons and permanent gases, including nitrogen, hydrogen, oxygen, carbon monoxide and carbon dioxide, within 6 minutes.

This application note describes a method for high-resolution analysis of refinery gas using an Agilent 7890A GC. The method uses a triple detector configuration, including an FID, a TCD (using nitrogen or argon as the carrier gas) and a TCD (using helium as the carrier gas). This method allows analysis of a variety of components, including permanent gases, hydrocarbons and sulfur compounds, in 6 minutes.

This application note describes a method for analyzing oxycodone and its metabolites (noroxycodone, oxymorphone, and noroxymorphone) in plasma using high-performance liquid chromatography/mass spectrometry (HPLC/MS) and the Agilent ZORBAX Rapid Resolution High Throughput (RRHT) StableBond SB-C18 column for chromatographic separation. The method employs an ammonium acetate/acetonitrile gradient and detection by electrospray positive ionization mode mass spectrometry. Spiked human plasma samples are subjected to solid-phase extraction prior to HPLC/MS analysis. The method provides fast, efficient analysis with minimal solvent consumption, which increases productivity, and good linearity (R2 > 0.9900) and reproducibility (less than 10% difference between replicate analyses) for all compounds.

The Agilent 7700x ICP-MS is an instrument for analyzing trace metals in drinking water. It features the third generation Octopole Reaction System (ORS3) technology and improved Helium (He) collision mode, which allows for the removal of polyatomic ion interferences without the need for complex interference correction equations. This results in easier method development, lower detection limits, and shorter analysis time.

This article introduces a method for analyzing the odor of mineral water using solid phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS). The samples were analyzed using multivariate analysis software (Mass Profiler Professional), and 26 components with statistically significant differences were selected. This method can extract components that are difficult to confirm through chromatogram comparison, making it useful for identifying the causes of odor.

This application note describes a method for simultaneous determination of 85 water quality regulated pesticides using Agilent GC/MS, including 76 organophosphorus pesticides, including 9 oxidation products and 5 oxidation products of fenitrothion. At a standard solution of 5 ppb, all pesticides can be detected, and the RSD (n=6) of the internal standard is 2.1-16.8%.

This document describes the features of the new PP&TT system Stratum, including low dead volume, high sensitivity analysis, and low carryover. When combined with Agilent's 7890A/5975CTAD system and CFT technology, efficient analysis can be achieved.

This document is a user guide for Agilent Technologies' Agilent G2565CA Scanner with SureScan high-resolution technology. It is intended for research purposes only and not for diagnostic procedures.

This manual describes the configuration of the Agilent G1978A Multi-mode Source.