Agilent Manuals

The Agilent 1260 Infinity Bio-inert Quaternary LC system has the same performance specifications as the standard Agilent 1260 Infinity Quaternary LC system based on the proven technology of the Agilent 1200 Infinity Series. This Technical Overview proves that method transfer from an Agilent 1260 Infinity Series Quaternary LC system to an Agilent 1260 Infinity Bio-inert Quaternary LC system will typically result in chromatograms with no significant differences including compatibility with standard methods with a maximum pressure of 600 bar. In addition, it is especially well suited for bio-analytical applications, such as, protein analysis using ion exchange chromatography (IEX) or size exclusion/gel filtration (SEC).

Agilent 4100 MP-AES provides a low-cost multi-element analysis technique based on a microwave-generated plasma that runs using only nitrogen as opposed to fl ammable gases such as acetylene. This improves laboratory safety, while reducing the diffi culties associated with sourcing and replacing fl ame AA gases — especially in remote areas. With excellent long-term stability, improved linear dynamic range, superior detection limits, and faster measurement compared with fl ame AA, the Agilent 4100 MP-AES is the perfect analysis tool for achieving your productivity and profi tability goals, with the lowest cost of analysis. Part of what makes the Agilent 4100 MP-AES such an easy system to work with is the Agilent MP Expert software that controls the instrument. With a familiar worksheet interface, automated method development, and software applets that automatically load pre-set method templates, the Agilent MP Expert software dramatically simplifi es analysis.

This document is a technical overview of the Agilent 1220 Infi nity LC System. The system can be used for analytical-scale purifi cation of compounds. It is easy to operate and has a flow rate of up to 10 mL/min. The Agilent 1260 Infi nity Analytical-scale Fraction Collector can be used to collect fractions based on time and peak baseline.

Agilent's MassHunter is a powerful, easy-to-learn software that provides a common platform across Agilent's LC/MS, GC/MS, and ICP-MS systems. It simplifies the transfer of skills across the laboratory with its common user interface and standard terminology. The latest revision of ICP-MS MassHunter software (G7201A, A.01.02) offers configuration, setup, and control of the Agilent 7700 Series ICP-MS hardware and accessories, as well as method setup, acquisition, data processing, and reporting through a modern, integrated, and intuitive user interface.

Agilent VGA 77 Vapor Generation Accessory is a precision instrument for rapid determination of mercury and other hydride forming elements.

This Technical Overview demonstrates the performance of the Agilent 1290 Infi nity Autosampler with increased injection volume capability up to 120 µL.

This document introduces Agilent Intelligent System Emulation Technology (ISET) technology, which can be used to achieve seamless method transfer from Agilent 1100/1200 Series LCs to Agilent 1290 Infi nity LC. Using ISET, the same chromatographic results can be achieved without any modifications to the instrument or changes to the original method. The document demonstrates through experiments that gradients run on an Agilent 1100 Series LC System, including typical linear gradients as well as gradients with dif- ferent slopes, steps, and isocratic parts, correlate almost 100% to gradient profi les created on the Agilent 1290 Infi nity LC System with ISET.

This document introduces the use of Agilent high efficiency columns with LC/MS

The Agilent Instrument Control Framework (ICF) enables other providers of LC data acquisition and processing software to simplify the development of the control of Agilent LC instrumentation. In this Technical Overview we demonstrate how Agilent ICF facilitates enhanced control of the Agilent 1290 Infi nity LC through Waters Empower chromatography data software versions 2 and 3. The combination of Agilent ICF and Waters Empower software provides easy access to advanced features of the 1290 Infi nity LC such as priming and purging the pump, external needle wash, and acquisition of all eight MWD and DAD signals.

This document describes how to control the Agilent 1220 Infinity LC system through Waters Empower chromatography data software using the Agilent Instrument Control Framework (ICF). The combination of Agilent ICF and Waters Empower software provides easy access to advanced features of the 1220 Infinity LC system, such as overlapped injections.

This document describes the Agilent BioSolids Pack, which is a repository for SSNMR sequences designed to investigate protein samples.

This Technical Overview shows the development of a chromatographic method for the analysis of paracetamol and its impurities using 1290 Infi nity LC System with ISET. Having developed the method it was transferred to an Agilent 1100 Series Quaternary LC System.

This document introduces a hybrid technology that integrates supercritical fluid chromatography and ultra-high performance liquid chromatography, which significantly improves the separation performance of complex mixtures.

This Technical Overview discusses the compatibility of Agilent Jet Stream thermal gradient focusing technology with CE/MS. The combination of capillary electrophoresis and mass spectrometry using the Agilent triple tube coaxial sheath-flow sprayer is widely used due to its simplicity, versatility, and robustness. The study shows that with the Jet Stream source and the latest version of the CE/MS sprayer, this approach can now also be used in combination with the proprietary Agilent Jet Stream thermal gradient focusing technology. The sprayer performed well on a mass spectrometer equipped with a conventional ESI source and showed slightly improved performance compared to the standard sprayer. When the sprayer was used with an Agilent Jet Stream source, equivalent or better results were obtained compared to a conventional ESI source.

This document introduces the effects of proteins and buffers on staining, denaturation, and electrophoresis when analyzing proteins with Agilent P200 ScreenTape