Agilent Manuals

This document presents a repeatability study of polypropylene analysis using Agilent PLgel MIXED-B columns and the Agilent PL-GPC 220 integrated, high temperature instrument. The system's ability is demonstrated through the analysis of six polypropylene injections. A commercial sample of polypropylene was prepared at 1.5 mg/mL using the PL-SP 260 sample preparation system with a dissolution temperature of 160 °C and a dissolution time of two hours. Six aliquots of the master batch solution were dispensed into the PL-GPC 220 autosampler vials and placed in the carousel where the hot zone temperature was 160 °C and the warm zone was 80 °C. Figure 1 shows an overlay of the raw data chromatograms obtained for the six consecutive injections of the sample. The data were analyzed against a polystyrene standards calibration using the provided Mark-Houwink parameters to obtain the polypropylene equivalent molecular weight averages shown in Table 1.

This document introduces the Agilent High Sensitivity DNA kit, which can be used for sizing, quantification and quality control of DNA fragments. The kit's sensitivity is up to pg/µL, and it can detect DNA fragments in the range of 50-7000bp.

This Application Note describes how to optimize a typical SFC separation by changing: • System backpressure • Modifi er concentration • Column temperature

This document provides an overview of preparative gel permeation chromatography, which improves the resolution of sample components by recycling the sample back through the column multiple times before fraction collection, thus conveniently minimizing cross-fraction contamination. The high column volume of the Agilent PLgel 10 µm 100 Å, 25 × 300 mm prep column facilitates recycle capability. Improved resolution after three cycles is observed.

Describes the importance of loading in preparative gel permeation chromatography (GPC) and how loading can be significantly increased for very low molecular weight materials such as epoxy resins.

This document introduces the application of Agilent MIXED gel technology in gel permeation chromatography and size exclusion chromatography columns. This technology allows different pore size gels to be blended together to create a column with a linear calibration suitable for a specific molecular weight range. Selectivity in GPC/SEC separations can be controlled by adjusting the column length and the pore size distribution of the packing material. Compared to individual pore size gels, MIXED gel packing provides a larger molecular weight range and better separation performance.

Agilent SurePrint Technology is content centered microarray design enabling speed and flexibility

This document introduces the advantages of Agilent NMR System's DirectDigital Receiver, including high accuracy and better signal-to-noise

This document describes the Agilent 1260 Infinity SFC/MS solution, which provides superior sensitivity by seamlessly interfacing to the Agilent 6100 Series LC/MS system

Agilent Salt Tolerant Cold Probe provides the highest sensitivity for lossy samples such as proteins in aqueous solutions containing high concentrations of buffer or salt

Agilent NMR System 13C CPMAS with the Agilent NMR System，introduces the advantages of using Agilent NMR System for 13C CPMAS. The experiment used Agilent 400 MHz NMR system and Agilent 3.2 mm T3 HFXY probe, and obtained the HMB test spectrum.

Agilent OneNMR probe is a new NMR probe that can simultaneously detect 13C and 1H, and its performance is excellent.

This document introduces a fast method for acquiring 2D and 3D data called Automatic Spectral Compression (ASCOM). ASCOM can use standard data processing methods to achieve very rapid acquisition of 2D and 3D data without losing information. This can improve 2D data by 2-5 times and 3D data by 4-25 times. This capability is standard in BioPack for experiments run on an Agilent NMR system.

This document presents a technical overview of optimizing ELSD conditions using PEG 400. By tuning the nebulizer temperature, evaporator temperature, and gas flow rate, the sensitivity of detection can be maximized.