Agilent Manuals

This document introduces the technical overview of combining gel permeation chromatography (GPC) with Fourier transform infrared (FT-IR) spectroscopy. A linear concentration response is required for FT-IR to be successfully employed in GPC. The coupling of these techniques allows the composition of copolymers and blends to be investigated, providing a wealth of information.

Describes how Agilent 356-LC RI Detector can be used for fast and automated detection of epoxy resins

This document describes the advantages and characteristics of the PL-SAX 1000Å material. This material is suitable for the analysis and purification of globular proteins, with the optimum pore size for maximum loading and low band broadening. PL-SAX is a hydrophilic strong anion exchange chromatographic packing material from Varian, Inc. The combination of the rigid macroporous polystyrene/divinylbenzene (PS/DVB) polymer matrix and chemically stable quaternized polyethyleneimine coating allows the analysis of biomolecules over a wide range of mobile phase conditions and pH. The physical stability of the media permits their use with high eluent flow rates and high speed gradients for very rapid separations. This excellent stability ensures both rapid equilibration between separations and the use of aggressive clean-up procedures employing high salt, NaOH, mineral and organic acids.

This document introduces the application of the Varian Evaporative Light Scattering Detector in the GPC analysis of polymer blends (and block copolymers).

This document introduces the response characteristics of RI and ELSD, and uses a series of polystyrene standards for research. The results show that the ELSD has higher sensitivity, but the relative peak areas of the two are very similar. In addition, the ELSD performs better at low molecular weights.

The Varian Evaporative Light Scattering Detector is a powerful tool for detecting any sample that is less volatile than the mobile phase in HPLC applications, irrespective of the optical properties of the compounds of interest. The ELSD offers distinct advantages over more conventional UV or DAD detection, particularly for gradient separation. In the past, one disadvantage for some ELSD designs has been that high operating temperatures required to fully evaporate the eluent can cause loss of semi-volatile, small molecules, e.g. drug candidates. The Varian Evaporative Light Scattering Detector features design improvements to overcome these drawbacks. For the detection of semi-volatile components in a sample, the Varian ELSD can be operated with the heaters off (ambient temperature operation) or with the nebulizer and evaporator controlled at low temperatures. Figure 1 shows a separation of a test mixture of four compounds by reversed phase HPLC, which is typically used to assess HPLC system performance for pharmaceutical analysis.

Varian Evaporative Light Scattering Detector is a technology that enhances LC-MS and can detect compounds that have little or no UV chromophore.

This document introduces the advantages of Varian OneNMR™ probe, including excellent lineshape performance, superior B1 field homogeneity and outstanding salt tolerance. The probe uses a solvent suppression technique called “Presaturation Utilizing Relaxation Gradients”, or PURGE, which has very efficient and robust solvent suppression effects, excellent frequency selectivity, and no baseline perturbations outside of the suppression window.

This document demonstrates the reproducibility and reliability of the Varian 320-MS triple quadrupole mass spectrometer in analyzing complex samples. By using the 212-LC binary gradient LC/MS chromatography pumps, the instrument can achieve excellent reproducibility of both area and retention time over multiple injections. The method was tested on a heavy matrix containing multiple components and transitions, showing excellent reliability and reproducibility of the system. The document also describes the specific steps and experimental conditions for analyzing samples extracted from tomato, orange, spinach, sweet corn, and raisin matrices using the QUECHERS method. The experimental results show that the % RSD of Buprofezin (306 ➔ 201) retention time is 0.02 and 0.09 over 60 and 100 subsequent injections, respectively, demonstrating the reliability of the system.

This document introduces Varian 320-MS, a triple quadrupole mass spectrometer with a unique 180° off-axis collision cell and triple quadrupole configuration. This instrument can eliminate chemical background noise and cross-talk when similar product ions in complex matrices are monitored in the same time segment.

Varian's 320-MS triple quadrupole system meets the quality guidelines for qualifier assessment and ion ratio stability set by the European Union for the detection and analysis of toxic compounds.

This is a research article that studied the performance of two polymeric strong cation exchange SPE products, the Bond Elut PCX and SampliQ SCX, to determine which product performed better in the retention of acidic, neutral, and basic components in human plasma.