Agilent Manuals

Introduces the performance improvements of the Agilent 1200 Series LC, including detection limits, analysis speed and solvent consumption, and provides experimental equipment configuration.

This document introduces how to reduce HPLC solvent consumption by using short LC columns with small internal diameters and particles to combat the worldwide shortage of acetonitrile.

This document provides detailed specifications for Agilent G3 microarray formats and tolerances

Agilent Jet Stream technology enhances sensitivity in ESI-MS by improving the desolvation and spatial focusing of ions. Super-heated nitrogen sheath gas effectively dries ions and concentrates them in a thermal confinement zone. Desolvation reduces noise. Full confinement of the spray by the super-heated nitrogen gas eliminates sample recirculation and reduces peak tailing.

This document describes the features and performance of the Velocity11 Bravo liquid handling platform, as well as the advantages of using this platform for automated liquid handling.

This document introduces the Agilent Bravo Automated Liquid Handling Platform 96-Channel 10 µL Tip Accuracy and Precision Technical Overview.

This document describes a method to measure the accuracy and precision of the Bravo in conjunction with a 96-Channel ST Disposable Tip Head. Measurements are determined by dispensing a tartrazine/DMSO solution into dry microplates, filling the plates with water, and measuring tartrazine absorbance. The product’s performance meets or exceeds CVs of 5% and accuracy of ±10% of the desired volume across the practical volume range.

The Agilent Bravo Automated Liquid Handling Platform is a liquid handling device with 384-channel 10 µL tip accuracy and precision. It offers high accuracy, high precision, and high throughput.

This document introduces the accuracy and precision technical overview of the Agilent Bravo Automated Liquid Handling Platform's 384-channel 30 µL tip

This document describes the accuracy and precision of 30μL 384-channel tips, which are stable and constant over 100 cycles of tip attachment and detachment. Accuracy and precision are nearly as good on the 100th liquid transfer as they are on the first.

This document introduces the accuracy and precision of the Agilent Bravo Automated Liquid Handling Platform 384-channel 70µL tip.

This document introduces a method for reducing HPLC solvent use and waste. By changing the HPLC column while keeping the same bonded phase, it is possible to realize a 40 to 90 percent reduction in acetonitrile usage. Possibilities include reducing the diameter of the column, changing to a shorter column with a smaller particle size, or a simultaneous reduction of length, diameter, and particle size. These options are easy to implement, do not require redevelopment of the method, and in some cases significantly reduce the analysis time.

Agilent Multimode Inlet is a gas chromatography inlet that can introduce a large amount of sample (5 µL to 250 µL or more) into Agilent 7890 Series GC systems, thereby reducing detection limits while maintaining high sample throughput and quality of results.

High-sensitivity detection cell for the Agilent Capillary Electrophoresis system represents a major advance in detection technology for capillary electrophoresis, increasing sensitivity by an order of magnitude.

cIEF is a method used to run on the Agilent Capillary Electrophoresis (CE) system. It can separate proteins and peptides according to differences in their isoelectric points (pI). The pI of a protein is an important characteristic and cIEF can be used to either determine a protein's pI or to perform separations based on pI differences. Separations based on pI are used in protein analysis for identification, purity assessment, degradation checking, microheterogeneity analysis, clinical diagnosis, and the analysis of complex protein mixtures. The cIEF method described here is a two step method. First, the capillary is filled with a mixture of protein sample and ampholytes which is then focused by applying a high field strength (V/cm). The ampholytes form a pH gradient in the capillary and the proteins are focused into sharp zones at the pH region which corresponds to their pI. In the second step the contents of the capillary are mobilized past the detector by apply- ing pressure to the inlet vial while maintaining the field strength. Focused peaks are monitored by the detector and appear in order from basic to acidic proteins. TEMED is used as a spacer between the outlet end of the capillary and the detec- tion point so that focusing of the proteins takes place between the capillary inlet and detectio