Agilent Manuals

This document describes the performance of the Agilent 1200 Series variable wavelength detector SL, including the use of different data rates and different detector cells.

The GC PAL and Combi PAL from CTC Analytics are flexible and expanded sample introduction tools that can meet the diverse application requirements in laboratories. These samplers offer a wide range of functionalities and customizable processes to accommodate specific situations. They support various liquid injection volumes, injection styles, and also enable automated solid-phase micro-extraction, heated headspace sampling, continuous flow sampling, and other approaches.

This document introduces the performance of the Agilent microarray platform in the MAQC study.

This paper evaluates the reproducibility of microarray technology and presents metrics for evaluating quality and reproducibility. The authors use data from the Agilent microarray system and Rosetta Resolver algorithms to demonstrate that the system produces data with high confidence for gene expression interpretations. The paper also provides metrics for calculating the reproducibility of microarray technology, including metrics for the microarray preparation process, signal statistics for each gene on each array, signal statistics for each gene across microarrays spotted with the same sample, log ratio statistics for all genes on each self-self microarray, log ratio statistics for each gene on each differential expression microarray, log ratio statistics for each gene across all differential expression microarrays, and biological replicate metrics.

This article introduces a new hybridization apparatus and method that improves the hybridization quality, consistency, and ease of use of the existing system. The apparatus improves hybridization quality by providing a hybridization chamber made from inert materials that enable easy hybridization buffer mixing. Consistency is improved through material quality and high-quality manufacturing. Finally, ease-of-use is enhanced through protocols that utilize standard lab equipment and a straightforward hybridization chamber assembly. The apparatus has been validated for use with Agilent's in-situ synthesized oligonucleotide microarrays and exhibits equivalent or superior experimental performance compared to Agilent's current standard hybridization method.

This Technical Note compares the Agilent 1100 Series diode array detector (DAD) “B” model to its successor the Agilent 1200 Series DAD “D model”. For this comparison both detectors were interchangeably connected to an Agilent 1100 Series LC system. To exclude undesired instrument bias the same Agilent 1100 Series LC system was used for both series of experiments. The evaluated performance criteria included retention time, peak width, resolution, peak area, peak height, signal-to-noise, limit of detection, limit of quantitation and linearity. Overall, the Agilent 1200 Series DAD compared favorably to the Agilent 1100 Series DAD, delivering the same or even noticeably better results. Based on these results it was concluded that an Agilent ChemStation method that was developed and validated on an 1100 Series DAD is fully compatible with the 1200 Series DAD. This means that an 1100 Series DAD can be replaced by a 1200 Series DAD with no or minimal revalidation effort.

This document introduces the Agilent 1200 Series Rapid Resolution LC system, which is the ideal instrument for very fast analysis up to 20 times faster than conventional HPLC with the same or better data quality in terms of resolution.

This overview describes a simple approach to cleaning and re-installing the 5973 and 5975 ion electron impact (EI) inert and standard sources and the chemical ionization (CI) source.

This document introduces the technical specifications of gas chromatograph detectors, including sensitivity, linearity, selectivity and repeatability. It also provides specific definitions and measurement methods for the relevant indicators, and finally summarizes the specific requirements for each indicator.

This overview provides guidance on implementing higher ion source temperatures for the 5975C series mass spectrometers and provides recommendations on how to choose an operating source temperature.

This manual introduces the auto-tuning process of Agilent 5975C series triple quadrupole mass spectrometer, including the concept of tuning target, tuning process, tuning result confirmation method, etc.

This is an article about gene expression database

This document introduces Agilent's GeneSpring GX EPI Programming software, including its R language interface, and how to integrate programs into GeneSpring GX

This Technical Note describes how to create a simple identification/calibration table in ChemStation.