Agilent Manuals (Industrial)
Agilent Narrow Bore vs. Standard Bore - Separation of Polypeptides
This document highlights the advantages and characteristics of narrow bore and standard bore separation of polypeptides. It mentions that narrow bore columns require only 20% of the mobile phase and 1/3 of the sample volume compared to standard bore columns under sample-limited conditions. It also emphasizes the need to minimize extra-column volume for optimal efficiency when using narrow bore columns.
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Agilent Stability-Test Comparison - ZORBAX Eclipse XDB-C8 vs. Symmetry-C8
This article compares the stability test results of ZORBAX Eclipse XDB-C8 and Symmetry-C8. The ZORBAX particle technology results in an extremely strong and dense silica support that dissolves more slowly at higher pH than xerogel particles used in Symmetry column packings. The extra dense bonding of ZORBAX XDB-C8 helps to shield the silica support at intermediate pH and decreases loss of chromatographic performance compared to Symmetry C8. By combining dense, durable particles, extra-dense bonding, and double endcapping, ZORBAX Eclipse packings (e.g., XDB-C8) have increased lifetime compared to Symmetry columns.
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Agilent Technologies Effect of Particle Size on Column Efficiency - ZORBAX 300SB-C8
The use of a column packing having reduced particle size can improve efficiency of a separation or, alternatively, increase loading capacity.
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Agilent Technologies Comparison of Micro Regular Flow Cell ZORBAX 300SB-C8 (3.5 m)
This document compares the use of micro and regular flow cells with ZORBAX 300SB-C8 (3.5 µm) column for peptide and protein analysis. It highlights the reduction of analysis time without compromising efficiency. By reducing the flow cell size, the effects of extra-column volume are minimized with small particle-size packing.
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Agilent Technologies Effect of eXtra-Dense Bonding Double End Capping on Peakshape
This article discusses the effect of Rx silica, eXtra-Dense Bonding, and double end capping on peak shape, as well as the improved chromatographic performance of Eclipse XDB column packings for basic molecules (P and A) at neutral pH. By reducing the interaction of basic molecules with the packing surface, good peak shape is achieved. ZORBAX XDB-C8 exhibits better peak shape than Symmetry C8, even for the strongly basic molecule amitriptyline.
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Agilent Technologies Use of Column Configuration to Increase Resolution Decrease Run Time
The use of smaller particle-size columns can increase resolution and peak height, while decreasing run time.
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Agilent Technologies OSHA Methods for Analysis Using ZORBAX HPLC Columns
This is a list of OSHA methods for analysis using Zorbax HPLC columns. These lists come from the OSHA web site www.osha-slc.gov/, under 'Chemical Sampling Information'. The document describes standard methods for analysis of many compounds using Zorbax HPLC columns.
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Agilent Technologies Benefits of Using Smaller-Diameter Columns
Using smaller-diameter columns can improve instrument sensitivity (peak response), save time and reduce solvent consumption
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Agilent It's Not the Initial Chromatogram It's the Column Lifetime Application Technical Robert Ricker
This document introduces the stability of ZORBAX Eclipse XDB-C8 column to neutral pH conditions, and verifies in experiments that ZORBAX Eclipse XDB-C8 column can be used for a longer time in neutral pH conditions.
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Agilent Technologies Effect of Bonded-Phase Functionality on the Recovery of a Synthetic Lipopeptide
This document introduces the features of Agilent's StableBond bonded phases, including low pH, high temperature compatibility, and a wide range.
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Agilent Technologies Preparative Separation of a Hydrophobic Peptide
This application note demonstrates the advantages of using high temperature and low pH conditions with ZORBAX StableBond C8 packing materials for high-efficiency preparative separations.
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Agilent Ultra-Fast Analyses using Rapid Resolution Cartridges
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Agilent Technologies Fast Gradient Analysis Fast Re-equilibration using LC/MS Columns
LC/MS columns have outstanding separation and fast equilibration time in short columns, small particles (3.5 vs. 5 µm), gradient mode.
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Agilent Technologies Distinct Selectivity of ZORBAX SB-Phenyl SB-C18 Bonded Phases
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Agilent Technologies Selectivity of the ZORBAX Eclipse Series
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Agilent Technologies Eclipse XDB Family of Bonded Designed for Optimum Lifetime at Intermediate pH
This article introduces the features and characteristics of Agilent ZORBAX Eclipse XDB series products. The series is designed for optimal lifetime at intermediate pH range.
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Agilent Technologies Function of Column Length in Fast Chromatography
This article describes the use of short column lengths for high performance liquid chromatography (HPLC) and discusses some of the advantages of this method. Short column lengths can reduce analysis times, increase peak heights, and allow for some tolerable loss in resolution. This method is particularly well suited for high-throughput analysis and can reduce solvent consumption and enhance sensitivity for mass-limited samples.
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Agilent Technologies Optimized Column Configuration Operating Parameters for High-Throughput Analyses
This document introduces the optimization of Agilent ZORBAX Eclipse XDB-C18 column for ultra-rapid HPLC separations. The parameters optimized include column, separation time, mobile-phase re-equilibration, and baseline drift.
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Agilent Effect of Mobile Phase Preparation on Chromatography
This document shows the effect of different mobile phase preparation methods on retention behavior in an Agilent 1100 LC system.
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Agilent Compound purification using the Agilent 1100 Series purification system with a mass-selective detector
This document describes the method and advantages of compound purification using the Agilent 1100 Series purification system. The use of a mass-selective detector enables mass-based fraction collection, enhancing and simplifying time- and peak-based fraction collection methods.
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