SL SL2072 RNaseA User Manual

About this manual

The document describes SL2072 RNaseA, a highly specific endonuclease derived from bovine pancreas. It specifically attacks the bond between the phosphate group at C'3 of pyrimidine nucleotides and the C'5 of adjacent nucleotides in RNA, producing 3' pyrimidine mononucleotides or low oligonucleotides ending in 3' pyrimidine nucleotides. The product is a 10mg/mL solution that has been treated to remove DNase activity and is used to degrade RNA during genomic DNA and plasmid extraction, as well as to remove RNA contamination during protein purification. It should be stored at 4°C for a shelf life of 3-6 months, or at -20°C for long-term storage. This manual is written in Chinese.

What's inside

• Product Introduction
• Storage Conditions
• Packaging Contents
• Usage Methods
• Plasmid Extraction

Specifications

Frequently asked questions

What is the recommended final concentration of RNaseA for plasmid extraction?

The recommended final concentration is 100μg/mL.

How much RNaseA solution should be added to 50μL of plasmid solution?

You should add 0.5μL of the RNaseA solution.

At what temperature and for how long should the mixture be incubated after adding RNaseA?

Incubate at 37℃ for 10 minutes.