Instructions for storage, handling, and use of SL3860 IPTG solution for protein expression and blue‑white screening.
The SL3860 IPTG solution is supplied as a sterile, filtered 50 mg/mL solution in 1 mL vials. It should be stored at –20 °C and remains usable for at least 12 months. The product is used as an inducer in recombinant protein expression and as part of blue‑white colony screening.
The manual provides preparation guidelines, including the amount of solution required to reach specific final concentrations (e.g., 0.5 mM in 1 L culture requires 2.38 mL of the 50 mg/mL solution) and instructions for incorporating IPTG and X‑gal into agar plates for screening. This manual is written in Chinese.
| Concentration | 50 mg/mL |
|---|---|
| Storage temperature | -20 °C |
| Shelf life | ≥12 months |
| Package volume | 1 mL |
| Molar concentration | 210 mM |
| Amount for 0.5 mM final (1 L) | 2.38 mL |
Store the IPTG solution at –20 °C.
The solution remains valid for at least 12 months.
The solution is supplied at 50 mg/mL.
Add 2.38 mL of the 50 mg/mL IPTG solution per liter of medium.