Technical datasheet for Beijing SiZhengBai Biotechnology's MRT6104-2 and MRT6104-5 cDNA first‑strand synthesis premix reagents, covering composition…
The cDNA first‑strand synthesis premix reagents (MRT6104-2, 20 rxn; MRT6104-5, 50 rxn) are high‑sensitivity RT‑Mix formulations designed for the first step of two‑step RT‑PCR. They enable synthesis of first‑strand cDNA from very low amounts of total RNA or poly(A) mRNA without adding separate RNase inhibitors, yielding longer cDNA up to 15 kb.
The datasheet provides detailed component lists, storage conditions (‑20 °C, one‑year shelf life), and step‑by‑step usage instructions, including RNA preparation, primer selection, incubation temperatures, and optional DNase/RNase treatments. Precautions and optional procedures are also described to ensure optimal cDNA yield and downstream PCR compatibility. This manual is written in Chinese.
| Model MRT6104-2 | 20 reactions |
|---|---|
| Storage condition | -20 °C, 1 year shelf life |
| RTMix溶液A volume (MRT6104-2) | 20 ml |
| RTMix溶液A volume (MRT6104-5) | 50 ml |
| 2×ReactionMix volume (MRT6104-2) | 200 ml |
| 2×ReactionMix volume (MRT6104-5) | 500 ml |
| Oligo(dT)18 (50 µM) volume (MRT6104-2) | 20 ml |
| Oligo(dT)18 (50 µM) volume (MRT6104-5) | 50 ml |
| RandomPrimer (50 µM) volume (MRT6104-2) | 20 ml |
| RandomPrimer (50 µM) volume (MRT6104-5) | 50 ml |
| DEPC‑ddH2O volume (MRT6104-2) | 1 ml |
Mix the RNA template with the chosen primer and DEPC‑ddH2O, incubate at 65 °C for 5 minutes, then place on ice for 2 minutes before adding the remaining reaction components.
Store the reagents at –20 °C; they remain stable for one year under these conditions.
The cDNA product should not exceed 10 % of the total PCR reaction volume to avoid affecting yield.