The manual provides storage conditions, component list, and step‑by‑step protocol for extracting total RNA from Gram‑positive and Gram‑negative bacteria…
The MRN1210-50 bacterial RNA extraction kit is designed for rapid isolation of total RNA from a wide range of Gram‑negative and Gram‑positive bacteria. The kit includes Solution A (30 ml), Solution B (20 ml), 50 spin‑adsorption columns, 50 ml universal wash buffer, 10 ml RNA elution buffer, and a user manual.
The protocol outlines preparation of fresh lysis solution (mix A and B 1:1), sample lysis, phase separation with chloroform, binding to the column, washing, and elution of RNA. Critical steps include centrifugation at 12 000‑15 000 g for specified times, careful handling of the aqueous phase to avoid DNA/protein contamination, and optional DNase treatment. Extracted RNA can be stored at –80 °C or used immediately for downstream applications. This manual is written in Chinese.
| Solution A volume | 30 ml |
|---|---|
| Solution B volume | 20 ml |
| Typical OD260/OD280 of extracted RNA | ≈2.0 |
| RNA purity range (OD260/OD280) | 1.8‑2.1 |
Mix Solution A and Solution B in a 1:1 ratio to make fresh lysis solution, and use it immediately.
Centrifuge at 12 000‑15 000 g at room temperature for 3‑5 minutes to bind the lysate to the column.
The RNA eluate can be used immediately or stored at –80 °C for later use.
Run the RNA on a formaldehyde‑denaturing agarose gel; non‑denaturing gels cannot separate all RNA molecules.