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Featured 北京四正柏生物科技有限公司 manuals
Beijing Sizhengbai Biotechnology Random Mutagenesis Kit MSM5304-20 User Manual
The Random Mutagenesis Kit (MSM5304-20) from Beijing Sizhengbai Biotechnology enables the introduction of random codon mutations using error‑prone PCR. The kit includes an optimized 2xMutRandomSystem, MutEnhancer, and ddH2O, allowing quick setup with DNA template and primers.
The manual describes storage conditions, reaction mixtures, primer design rules, PCR cycling parameters, and methods to control mutation rates by adjusting MutEnhancer volume, template concentration, and cycle number. Safety notes and troubleshooting tips are also provided. This manual is written in Chinese.
Covers: Product Description · Storage Conditions · Kit Components · Primer Design Guidelines · PCR Reaction Setup · Mutation Rate Control
4ABio MRN1217-50 miRNA Extraction Kit Technical Data Sheet
The MRN1217-50 kit is a column‑based, one‑step solution for rapid purification of small RNAs (miRNA, siRNA, etc.) from animal tissue, blood, plant material and other samples. It offers high purity (OD260/280 > 1.9) and yields of 20‑40 µg RNA per 100 mg animal tissue or 10‑30 µg per 100 mg plant tissue, with no genomic DNA contamination.
The protocol includes preparation of a fresh lysis solution (mixing solutions A and B 1:1), centrifugation steps to remove large RNA and DNA, and final elution of miRNA in RNase‑free water. The kit components include solutions A, B, C, a centrifuge adsorption column, wash buffer and RNA elution buffer, sufficient for 50 extractions. This manual is written in Chinese.
Covers: Product Description · Storage Conditions · Specifications and Components · Usage Method · Appendix
北京四正柏生物科技有限公司 MRT6104-2 / MRT6104-5 cDNA First Strand Synthesis Premix Reagent Technical Datasheet
The cDNA first‑strand synthesis premix reagents (MRT6104-2, 20 rxn; MRT6104-5, 50 rxn) are high‑sensitivity RT‑Mix formulations designed for the first step of two‑step RT‑PCR. They enable synthesis of first‑strand cDNA from very low amounts of total RNA or poly(A) mRNA without adding separate RNase inhibitors, yielding longer cDNA up to 15 kb.
The datasheet provides detailed component lists, storage conditions (‑20 °C, one‑year shelf life), and step‑by‑step usage instructions, including RNA preparation, primer selection, incubation temperatures, and optional DNase/RNase treatments. Precautions and optional procedures are also described to ensure optimal cDNA yield and downstream PCR compatibility. This manual is written in Chinese.
Covers: Product Description · Product Components · Precautions · Operation Procedure · Additional Notes
Latest 北京四正柏生物科技有限公司 manuals
Beijing Sizhengbai Biotechnology Random Mutagenesis Kit MSM5304-20 User Manual
User Manual 3 pages · 330 KB · ZHThe MSM5304-20 Random Mutagenesis Kit manual provides storage instructions, component details, and step‑by‑step error‑prone PCR guidelines.
4ABio MRN1217-50 miRNA Extraction Kit Technical Data Sheet
Product Leaflet 3 pages · 233 KB · ZHTechnical data sheet for the 4ABio MRN1217-50 miRNA extraction kit, describing features, storage, yield and step‑by‑step usage.
北京四正柏生物科技有限公司 MRT6104-2 / MRT6104-5 cDNA First Strand Synthesis Premix Reagent Technical Datasheet
Product Leaflet 3 pages · 269 KB · ZHTechnical datasheet for Beijing SiZhengBai Biotechnology's MRT6104-2 and MRT6104-5 cDNA first‑strand synthesis premix reagents, covering composition…
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in 北京四正柏生物科技有限公司 manuals
How should the kit be stored?
Store the kit at –20 °C for up to one year, or at 4 °C for up to three months if used frequently.
From: Beijing Sizhengbai Biotechnology Random Mutagenesis Kit MSM5304-20 User Manual
How many reactions does the kit provide?
The kit supplies reagents for 20 reactions.
From: Beijing Sizhengbai Biotechnology Random Mutagenesis Kit MSM5304-20 User Manual
What is the recommended storage temperature for the kit?
Store the kit at 4 °C (after room‑temperature transport); the shelf life is one year.
From: 4ABio MRN1217-50 miRNA Extraction Kit Technical Data Sheet
How much RNA can be obtained from 100 mg of animal tissue?
Typical yield is 20‑40 µg of small RNA per 100 mg of animal tissue.
From: 4ABio MRN1217-50 miRNA Extraction Kit Technical Data Sheet
How should the RNA template be prepared before cDNA synthesis?
Mix the RNA template with the chosen primer and DEPC‑ddH2O, incubate at 65 °C for 5 minutes, then place on ice for 2 minutes before adding the remaining reaction components.
From: 北京四正柏生物科技有限公司 MRT6104-2 / MRT6104-5 cDNA First Strand Synthesis Premix Reagent Technical Datasheet
What are the recommended storage conditions for the premix reagents?
Store the reagents at –20 °C; they remain stable for one year under these conditions.
From: 北京四正柏生物科技有限公司 MRT6104-2 / MRT6104-5 cDNA First Strand Synthesis Premix Reagent Technical Datasheet