The MSM5304-20 Random Mutagenesis Kit manual provides storage instructions, component details, and step‑by‑step error‑prone PCR guidelines.
The Random Mutagenesis Kit (MSM5304-20) from Beijing Sizhengbai Biotechnology enables the introduction of random codon mutations using error‑prone PCR. The kit includes an optimized 2xMutRandomSystem, MutEnhancer, and ddH2O, allowing quick setup with DNA template and primers.
The manual describes storage conditions, reaction mixtures, primer design rules, PCR cycling parameters, and methods to control mutation rates by adjusting MutEnhancer volume, template concentration, and cycle number. Safety notes and troubleshooting tips are also provided. This manual is written in Chinese.
| Storage temperature | -20 °C (1 year) or 4 °C (3 months) |
|---|---|
| Number of reactions | 20 reactions |
| Kit components | 2xMutRandomSystem, MutEnhancer, ddH2O |
Store the kit at –20 °C for up to one year, or at 4 °C for up to three months if used frequently.
The kit supplies reagents for 20 reactions.
Mutation rate can be tuned by changing the volume of MutEnhancer, the concentration of the DNA template, and the number of PCR cycles.