Product leaflet for the Bioline SensiFAST SYBR No-ROX Kit, a real-time PCR mastermix optimized for fast, specific, and sensitive amplification without a…
The SensiFAST SYBR No-ROX Kit is a 2x mastermix designed for fast, highly reproducible, and sensitive real-time PCR. It contains all necessary components including SYBR Green I dye, dNTPs, stabilisers, and enhancers, requiring only the addition of primers and template. The kit is shipped on dry/blue ice and must be stored at -20°C.
| Reaction Volume (500 pack) | 500 x 20 µL reactions |
|---|---|
| Reaction Volume (2000 pack) | 2000 x 20 µL reactions |
| Mastermix Concentration | 2x |
| Recommended Primer Tm | approximately 60 °C |
| Optimal Amplicon Length | 80 -200 bp |
| Recommended Primer Concentration | 400 nM |
| MgCl2 Concentration | 3 mM |
All kit components should be stored at -20°C upon receipt.
No, it is designed for instruments that do not need a passive reference signal.
Up to 1 µg of complex genomic DNA is recommended for a single PCR.