This document is the user manual of JetSeq™ DNA Library Preparation Kit, which introduces the composition, usage methods and precautions of the product.
The Bioline JetSeq™ DNA Library Preparation Kit is designed to generate high-quality next-generation sequencing (NGS) libraries suitable for Illumina MiniSeq, MiSeq, NextSeq, or HiSeq instruments. The kit contains all necessary enzymes and buffers for end-repair, A-tailing, ligation, and amplification in master mix formulations, along with 16 barcoded adapters.
| Compatible instruments | Illumina MiniSeq, MiSeq, NextSeq or HiSeq |
|---|---|
| Low input requirement | 1 ng - 3 µg fragmented DNA |
| Preparation time | Under 3 hours |
| Number of barcoded adapters | 16 |
The kit has been developed and optimized for whole genome sequencing, exome sequencing, targeted sequencing, de novo sequencing, and ChIP sequencing.
The kit components should be stored at -20 °C to retain full activity until the expiry date.
Users need a thermal cycler or heat block and equipment such as Nanodrop, Qubit, Tapestation, Bioanalyzer, or equivalent.