This document introduces Bioline SensiFAST TM qPCR technology, which is a method for rapid detection of gene expression.
The Bioline SensiFAST qPCR Guide provides technical information on quantitative PCR (qPCR) techniques, emphasizing the importance of experimental design and adherence to MIQE guidelines for accurate gene expression analysis. It serves as a resource for researchers utilizing various SensiFAST kits for diagnostic, agricultural, biotechnology, and pharmaceutical research applications.
The document details the features and benefits of SensiFAST One-Step and Two-Step qPCR kits, highlighting their speed, sensitivity, and reproducibility. It includes specific product information for kits such as the SensiFAST SYBR One-Step Kit, SensiFAST Probe One-Step Kit, and SensiFAST cDNA Synthesis Kit, along with a comprehensive table listing compatible qPCR instruments from manufacturers like Agilent, Applied Biosystems, Bio-Rad, and Roche.
| Cycling Time | as little as 40 minutes |
|---|---|
| Dynamic Range | 5 orders of magnitude |
| Sensitivity | down to 10 copies per reaction |
qPCR allows you to determine the starting template copy number of your DNA or cDNA with accuracy and high sensitivity over a wide dynamic range.
SensiFAST One-Step Kits can be used on all qPCR instruments, including models from Agilent, Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Fluidigm, Qiagen, Roche, Takara, and Thermo.