Key facts
- Manuals
- 4
- Languages
- EN
- Last added
- 2023-10-16
Featured Bioline manuals
Bioline SensiFAST qPCR Guide
The Bioline SensiFAST qPCR Guide provides technical information on quantitative PCR (qPCR) techniques, emphasizing the importance of experimental design and adherence to MIQE guidelines for accurate gene expression analysis. It serves as a resource for researchers utilizing various SensiFAST kits for diagnostic, agricultural, biotechnology, and pharmaceutical research applications.
The document details the features and benefits of SensiFAST One-Step and Two-Step qPCR kits, highlighting their speed, sensitivity, and reproducibility. It includes specific product information for kits such as the SensiFAST SYBR One-Step Kit, SensiFAST Probe One-Step Kit, and SensiFAST cDNA Synthesis Kit, along with a comprehensive table listing compatible qPCR instruments from manufacturers like Agilent, Applied Biosystems, Bio-Rad, and Roche.
Covers: Quantitative PCR (qPCR) · Gene Expression Analysis · SensiFAST One-Step Kits · SensiFAST Two-Step Kits · Instrument Compatibility · MIQE Guidelines
Bioline JetSeq DNA Library Preparation Kit User Manual
The Bioline JetSeq™ DNA Library Preparation Kit is designed to generate high-quality next-generation sequencing (NGS) libraries suitable for Illumina MiniSeq, MiSeq, NextSeq, or HiSeq instruments. The kit contains all necessary enzymes and buffers for end-repair, A-tailing, ligation, and amplification in master mix formulations, along with 16 barcoded adapters.
Covers: Kit contents · Description · Storage · Safety information · Product specifications · Equipment and reagents to be supplied by user
Bioline JetSeq Flex DNA Library Preparation Kit Product Manual
The Bioline JetSeq Flex DNA Library Preparation Kit is designed to generate high-quality next-generation sequencing (NGS) libraries suitable for Illumina MiniSeq, MiSeq, NextSeq, or HiSeq instruments. The kit combines end-repair and A-tailing into a single step to streamline the workflow and reduce variability. It includes all necessary enzymes and buffers for end-repair, A-tailing, ligation, and amplification, allowing users to use their own adapters.
Covers: Kit contents · Description · Storage · Safety information · Product specifications · Equipment and reagents to be supplied by user
Bioline JetSeq ER and Ligation Kit Product Manual
The Bioline JetSeq ER and Ligation Kit is designed to generate high-quality next-generation sequencing (NGS) libraries suitable for sequencing on Illumina instruments. The kit contains all necessary enzymes and buffers for end-repair, A-tailing, and ligation in convenient master mix formulations, offering a streamlined workflow that can produce adapter-ligated libraries in under 2 hours.
Covers: Kit contents · Description · Storage · Safety information · Product specifications · Equipment and reagents to be supplied by user
Latest Bioline manuals
Bioline SensiFAST qPCR Guide
Product Leaflet 15 pages · 2 MB · ENA guide for Bioline SensiFAST qPCR kits covering one-step and two-step protocols, instrument compatibility, and applications for gene expression analysis.
Bioline JetSeq DNA Library Preparation Kit User Manual
User Manual 27 pages · 832 KB · ENThis manual provides instructions for using the Bioline JetSeq DNA Library Preparation Kit to generate high-quality NGS libraries compatible with Illumina…
Bioline JetSeq Flex DNA Library Preparation Kit Product Manual
User Manual 21 pages · 618 KB · ENThis manual provides operating instructions for the Bioline JetSeq Flex DNA Library Preparation Kit, detailing protocols for NGS library generation…
Bioline JetSeq ER and Ligation Kit Product Manual
User Manual 14 pages · 271 KB · ENThis manual provides instructions for using the Bioline JetSeq ER and Ligation Kit to prepare high-quality NGS libraries compatible with Illumina platforms.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in Bioline manuals
What are the main advantages of qPCR over traditional end-point PCR?
qPCR allows you to determine the starting template copy number of your DNA or cDNA with accuracy and high sensitivity over a wide dynamic range.
Which instruments are compatible with SensiFAST One-Step Kits?
SensiFAST One-Step Kits can be used on all qPCR instruments, including models from Agilent, Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Fluidigm, Qiagen, Roche, Takara, and Thermo.
What applications is the JetSeq kit optimized for?
The kit has been developed and optimized for whole genome sequencing, exome sequencing, targeted sequencing, de novo sequencing, and ChIP sequencing.
From: Bioline JetSeq DNA Library Preparation Kit User Manual
What is the recommended storage temperature?
The kit components should be stored at -20 °C to retain full activity until the expiry date.
From: Bioline JetSeq DNA Library Preparation Kit User Manual
What equipment is required for library analysis?
Equipment for determining DNA concentration (e.g., Nanodrop, Qubit) and size distribution (e.g., Tapestation, Bioanalyzer) is required.
From: Bioline JetSeq Flex DNA Library Preparation Kit Product Manual
Can I use my own adapters?
Yes, the kit offers flexibility where users are free to use adapters of their choice.
From: Bioline JetSeq Flex DNA Library Preparation Kit Product Manual